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Figure 3 | Filaria Journal

Figure 3

From: Obligatory symbiotic Wolbachia endobacteria are absent from Loa loa

Figure 3

Cross sections of Onchocerca and L. loa stained with three antisera against Wolbachia. (A, B) Antiserum against Wol-Ov-AAT labels Wolbachia endobacteria (arrow-head in A) in a female O. volvulus but no bacteria are seen in a female L. loa, although sperms in the uterus are well labelled. (C, D) Antiserum against Y-HSP60 stains Wolbachia in the hypodermis of all sections of a female O. volvulus in an onchocercoma (arrow-head in C) but no bacteria can be detected in hypodermis or embryos of a female L. loa (D). (E, F) Antiserum against Wol-Di-WSP stains the bacteria in the lateral cords (arrows) of a male O. dukei (E). No bacteria are seen in the lateral or median cords of a male L. loa (F). lc, lateral cord; m, morulae: mc, median cord; n, neutrophil granulocytes; o, oocytes in the uterus; sp, sperms; u, uterus; scale bar = 50 μm.

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