Figure 5
From: Obligatory symbiotic Wolbachia endobacteria are absent from Loa loa
Oocytes and young morulae of Onchocerca and L. loa stained with three antisera against Wolbachia. (A, B) Antiserum against Wol-Di-WSP stains the Wolbachia (arrowhead in A) in all primary oocytes in the ovary of O. volvulus whereas no bacteria are seen in the oocytes in the ovary or in the morulae of a L. loa female (B). (C, D) Antiserum against Wol-Ov-AAT stains the Wolbachia (arrow-heads in C) in all mature oocytes in the uterus of O. ochengi but no bacteria are seen in the uterus of L. loa. The L. loa worm indicates its reactivity to the antiserum by well-labelled sperms. (E, F) Antiserum against Y-HSP60 stains the Wolbachia (arrow-head in E) in the lateral cord and in all young morulae of an O. gibsoni female but no bacteria can be detected in the morulae or in the lateral cord of a L. loa female (F). lc, lateral cord; m, morulae; o, oocytes; sp, sperms; scale bar = 25μm.