Figure 1
Detection of Wolbachia in Loa loa by PCR. A) Primers for eubacterial 16S rDNA only amplify a signal in Onchocerca volvulus positive controls (lanes 9 and 10). B) Primers for Wolbachia ftsZ only amplify DNA from O. volvulus. Lane description for A+B: 1–3: L. loa female worms, lanes 4–7: L. loa male worms, lane 8: L. loa microfilariae, lanes 9–10: O. volvulus, lanes 11–12: Acanthocheilonema viteae. C) Elongase® Taq, having 3'–5' proofreading activity, only detects endosymbiont DNA in the O. volvulus controls. Lanes 1–3: L. loa female worms, lanes 4–7:L. loa male worms, lanes 8–9: O. volvulus.