Figure 2

Expression pattern of the Av17promoter::GFP construct in transgenic Caenorhabditis elegans. (A) Fluorescence staining in the pharynx of a worm transformed with the Av17promoter::GFP fusion construct. The pharynx contains two classes of gland cells, g1 (two cells) and g2 (two cells) in the second bulb of the pharynx. The g1 cells extend three cuticle-lined ducts anteriorly within the narrow pharyngeal nerve cords, two of which pass through the isthmus and empty into the pharyngeal lumen near the first bulb and the dorsal g1 duct empties near the anterior end of the pharynx. The g2 cells extend shorter ducts, which empty into the lumen of the second bulb. (C) Fluorescence staining in the rectal gland cells (rectD, rectVL, rectVR) in the posterior of the same worm as in (A). These cells connect to the intestinal lumen just posterior to the rectal valve. (B) and (D) Bright field microscopy of (A) and (C), respectively.