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Table 3 Results for Wuchereria bancrofti DNA extraction in pools of laboratory-reared uninfected mosquitoes added (positive) or not (negative) with one experimentally infected mosquito.

From: PCR and dissection as tools to monitor filarial infection of Aedes polynesiensismosquitoes in French Polynesia

Pool size Method A (Reference) DNA sample fraction 191 bp band 225 bp band Résults *
1 positive controls 1/20e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) + (n = 1) - (n = 1)
20 positive controls 1/20e + (n = 2/3) + (n = 3/3) + (n = 2/3)
  negative control 1/20e - (n = 1) + (n = 1) - (n = 1)
30 positive controls 1/20e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) + (n = 1) - (n = 1)
50 positive controls 1/20e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) + (n = 1) - (n = 1)
Pool size Method B (Qiagen kit) DNA sample fraction 191 bp band 225 bp band Résults
1 positive controls 1/20e + (n = 3/3) + (n = 3/3) + (n = 3/3)
   1/40e + (n = 3/3) + (n = 3/3) + (n = 3/3)
   1/240e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) + (n = 1) - (n = 1)
   1/40e - (n = 1) + (n = 1) - (n = 1)
   1/240e - (n = 1) + (n = 1) - (n = 1)
20 positive controls 1/20e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/40e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/240e + (n = 2/3) + (n = 2/3) inh (n = 1/3)
   1/1200e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) - (n = 1) inh (n = 1)
   1/40e - (n = 1) - (n = 1) inh (n = 1)
   1/240e - (n = 1) - (n = 1) inh (n = 1)
   1/1200e - (n = 1) + (n = 1) - (n = 1)
30 positive controls 1/20e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/40e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/240e + (n = 2/3) + (n = 3/3) + (n = 2/3)
  negative control 1/20e - (n = 1) - (n = 1) inh (n = 1)
   1/40e - (n = 1) - (n = 1) inh (n = 1)
   1/240e - (n = 1) + (n = 1) - (n = 1)
50 positive controls 1/20e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/40e - (n = 3/3) - (n = 3/3) inh (n = 3/3)
   1/240e + (n = 3/3) + (n = 3/3) + (n = 3/3)
  negative control 1/20e - (n = 1) - (n = 1) inh (n = 1)
   1/40e - (n = 1) - (n = 1) inh (n = 1)
   1/240e - (n = 1) + (n = 1) - (n = 1)