Table 3 Results for Wuchereria bancrofti DNA extraction in pools of laboratory-reared uninfected mosquitoes added (positive) or not (negative) with one experimentally infected mosquito.
Pool size | Method A (Reference) | DNA sample fraction | 191 bp band | 225 bp band | Résults * |
|---|---|---|---|---|---|
1 | positive controls | 1/20e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) |
negative control | 1/20e | - (n = 1) | + (n = 1) | - (n = 1) | |
20 | positive controls | 1/20e | + (n = 2/3) | + (n = 3/3) | + (n = 2/3) |
negative control | 1/20e | - (n = 1) | + (n = 1) | - (n = 1) | |
30 | positive controls | 1/20e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) |
negative control | 1/20e | - (n = 1) | + (n = 1) | - (n = 1) | |
50 | positive controls | 1/20e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) |
negative control | 1/20e | - (n = 1) | + (n = 1) | - (n = 1) | |
Pool size | Method B (Qiagen kit) | DNA sample fraction | 191 bp band | 225 bp band | Résults |
1 | positive controls | 1/20e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) |
1/40e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) | ||
1/240e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) | ||
negative control | 1/20e | - (n = 1) | + (n = 1) | - (n = 1) | |
1/40e | - (n = 1) | + (n = 1) | - (n = 1) | ||
1/240e | - (n = 1) | + (n = 1) | - (n = 1) | ||
20 | positive controls | 1/20e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) |
1/40e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) | ||
1/240e | + (n = 2/3) | + (n = 2/3) | inh (n = 1/3) | ||
1/1200e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) | ||
negative control | 1/20e | - (n = 1) | - (n = 1) | inh (n = 1) | |
1/40e | - (n = 1) | - (n = 1) | inh (n = 1) | ||
1/240e | - (n = 1) | - (n = 1) | inh (n = 1) | ||
1/1200e | - (n = 1) | + (n = 1) | - (n = 1) | ||
30 | positive controls | 1/20e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) |
1/40e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) | ||
1/240e | + (n = 2/3) | + (n = 3/3) | + (n = 2/3) | ||
negative control | 1/20e | - (n = 1) | - (n = 1) | inh (n = 1) | |
1/40e | - (n = 1) | - (n = 1) | inh (n = 1) | ||
1/240e | - (n = 1) | + (n = 1) | - (n = 1) | ||
50 | positive controls | 1/20e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) |
1/40e | - (n = 3/3) | - (n = 3/3) | inh (n = 3/3) | ||
1/240e | + (n = 3/3) | + (n = 3/3) | + (n = 3/3) | ||
negative control | 1/20e | - (n = 1) | - (n = 1) | inh (n = 1) | |
1/40e | - (n = 1) | - (n = 1) | inh (n = 1) | ||
1/240e | - (n = 1) | + (n = 1) | - (n = 1) |