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Table 4 PCR amplification of Wuchereria bancrofti DNA in pools of laboratory-reared uninfected mosquitoes spiked with microfilariae

From: PCR and dissection as tools to monitor filarial infection of Aedes polynesiensismosquitoes in French Polynesia

Pool size

Control testing

191 bp band

225 bp band

Résults

0

Mf spiked controls

+ (n = 3/3)

+ (n = 3/3)

+ (n = 3/3)

 

Negative control

- (n = 1)

+ (n = 1)

- (n = 1)

20

Mf spiked controls

+ (n = 3/3)

+ (n = 3/3)

+ (n = 3/3)

 

Negative control

- (n = 1)

+ (n = 1)

- (n = 1)

30

Mf spiked controls

+ (n = 3/3)

+ (n = 3/3)

+ (n = 3/3)

 

Negative control

- (n = 1)

+ (n = 1)

- (n = 1)

50

Mf spiked controls

+ (n = 3/3)

+ (n = 3/3)

+ (n = 3/3)

 

Negative control

- (n = 1)

+ (n = 1)

- (n = 1)

  1. * DNA extracted according the B method; sample fraction used 1/240e