Skip to main content

Table 1 Summary of long-term trial 1: parasite mean motility scores and viability (MTT colorimetry)

From: Onchocerca parasites and Wolbachia endosymbionts: evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro

Compound/drug and conc. Mean motility scores over a period of 40 days Motility MTT
DAY 1 5 10 15 20 25 30 35 40 % reduction on day 40 (P) % inhibition on day 40 (P)
Control 8.0 7.4 7.1 7.0 5.3 5.5 6.3 6.4 4.9   
CGP 6140 1.25 × 10-5M (positive control) 0.5 0.8 0.0 0.5 0.0 0.0 0.0 0.0 0.0 100.0 (0.005) 83.3 (0.098)
Rifampicin 5 × 10-5M 7.7 6.8 6.7 6.8 3.2 1.7 0.2 0.2 0.0 100.0 (0.002) 89.6 (0.033)
Rifampicin 1.25 × 10-5M 7.0 7.0 7.3 5.7 6.5 5.2 5.7 5.0 5.6 0.0 (0.647) 0.0 (0.798)
Minocycline 5 × 10-5M 8.0 6.7 7.3 5.7 0.0 0.0 0.0 0.0 0.0 100.0 (0.002) 84.1 (0.042)
Minocycline 1.25 × 10-5M 7.8 7.5 6.7 5.7 4.3 2.5 4.8 1.7 0.0 100.0 (0.002) 93.7 (0.026)
Doxycycline 5 × 10-5M 8.3 8.0 8.0 5.7 0.3 0.0 0.0 0.0 0.0 100.0 (0.002) 93.0 (0.027)
Doxycycline 1.25 × 10-5M 7.3 6.5 6.3 4.8 6.3 6.3 6.2 6.2 5.5 0.0 (0.699) 0.0 (0.801)
Ethambutol 5 × 10-5M 6.8 6.8 6.5 6.7 4.0 4.8 4.3 5.0 2.7 44.9 (0.170) 73.0 (0.108)
Dapsone 5 × 10-5M 7.3 7.3 7.7 5.0 6.8 7.0 7.2 7.3 6.5 0.0 (0.250) 0.0 (0.798)
Pyrazinamide 5 × 10-5M 7.7 7.7 7.2 3.5 5.5 5.5 5.7 5.2 3.7 24.5 (0.503) 4.8 (0.949)
  1. The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.