Onchocerca parasites and Wolbachia endosymbionts: evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro

Table 2 Summary of long-term trial 2: parasite mean motility scores and viability (MTT colorimetry)

Compound/drug and conc.	Mean motility scores over a period of 40 days									Motility	MTT
DAY	1	5	10	15	20	25	30	35	40	% reduction on day 40 (P)	% inhibition on day 40 (P)
Control	7.6	7.2	7.1	6.8	5.9	4.9	5.8	6.1	6.3
^aCGP 6140 1.25 × 10^-5M (positive control)	1.3	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	100.0 (ND)	87.2 (ND)
^bNorfloxacin 5 × 10^-5M	6.9	7.4	7.1	2.4	2.5	2.6	1.4	1.0	2.5	60.3 (ND)	90.7 (ND)
Ciprofloxacin 5 × 10^-5M	6.9	7.9	7.1	3.6	4.1	3.0	2.3	2.5	3.0	52.4 (0.080)	73.3 (0.040)
Vancomycin 5 × 10^-5M	6.4	7.4	6.5	3.6	3.6	2.8	2.4	1.6	2.1	66.7 (0.009)	83.7 (0.022)
Gentamycin 5 × 10^-5M	7.6	5.5	6.3	2.8	2.6	0.8	1.5	1.0	1.6	74.6 (0.005)	83.7 (0.017)
Ceftriaxone 5 × 10^-5M	7.1	6.8	7.0	2.4	2.0	0.9	0.6	0.6	0.7	88.9 (<0.001)	90.7 (0.011)
^cTriclosan 5 × 10^-5M	6.9	4.9	0.4	0.1	0.0	0.0	0.0	0.0	0.0	100.0 (ND)	98.8 (ND)
^cCerulenin 5 × 10^-5M	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	100.0 (ND)	100.0 (ND)

a. Day 40 results based on 1 worm; 5 worms lost to microbial contamination
b. Day 40 results based on 2 worms; 4 worms lost to microbial contamination
c. Denotes compounds that were toxic to the monkey kidney cell feeder layer
The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.