|
Compound/drug and conc.
|
Mean motility scores over a period of 40 days
|
Motility
|
MTT
|
|---|
|
DAY
|
1
|
5
|
10
|
15
|
20
|
25
|
30
|
35
|
40
|
% reduction on day 40 (P)
|
% inhibition on day 40 (P)
|
|---|
|
Control
|
7.6
|
7.2
|
7.1
|
6.8
|
5.9
|
4.9
|
5.8
|
6.1
|
6.3
| | |
|
aCGP 6140 1.25 × 10-5M (positive control)
|
1.3
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
100.0 (ND)
|
87.2 (ND)
|
|
bNorfloxacin 5 × 10-5M
|
6.9
|
7.4
|
7.1
|
2.4
|
2.5
|
2.6
|
1.4
|
1.0
|
2.5
|
60.3 (ND)
|
90.7 (ND)
|
|
Ciprofloxacin 5 × 10-5M
|
6.9
|
7.9
|
7.1
|
3.6
|
4.1
|
3.0
|
2.3
|
2.5
|
3.0
|
52.4 (0.080)
|
73.3 (0.040)
|
|
Vancomycin 5 × 10-5M
|
6.4
|
7.4
|
6.5
|
3.6
|
3.6
|
2.8
|
2.4
|
1.6
|
2.1
|
66.7 (0.009)
|
83.7 (0.022)
|
|
Gentamycin 5 × 10-5M
|
7.6
|
5.5
|
6.3
|
2.8
|
2.6
|
0.8
|
1.5
|
1.0
|
1.6
|
74.6 (0.005)
|
83.7 (0.017)
|
|
Ceftriaxone 5 × 10-5M
|
7.1
|
6.8
|
7.0
|
2.4
|
2.0
|
0.9
|
0.6
|
0.6
|
0.7
|
88.9 (<0.001)
|
90.7 (0.011)
|
|
cTriclosan 5 × 10-5M
|
6.9
|
4.9
|
0.4
|
0.1
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
100.0 (ND)
|
98.8 (ND)
|
|
cCerulenin 5 × 10-5M
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
100.0 (ND)
|
100.0 (ND)
|
- a. Day 40 results based on 1 worm; 5 worms lost to microbial contamination
- b. Day 40 results based on 2 worms; 4 worms lost to microbial contamination
- c. Denotes compounds that were toxic to the monkey kidney cell feeder layer
- The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.
