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Table 2 Summary of long-term trial 2: parasite mean motility scores and viability (MTT colorimetry)

From: Onchocerca parasites and Wolbachia endosymbionts: evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro

Compound/drug and conc. Mean motility scores over a period of 40 days Motility MTT
DAY 1 5 10 15 20 25 30 35 40 % reduction on day 40 (P) % inhibition on day 40 (P)
Control 7.6 7.2 7.1 6.8 5.9 4.9 5.8 6.1 6.3   
aCGP 6140 1.25 × 10-5M (positive control) 1.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.0 (ND) 87.2 (ND)
bNorfloxacin 5 × 10-5M 6.9 7.4 7.1 2.4 2.5 2.6 1.4 1.0 2.5 60.3 (ND) 90.7 (ND)
Ciprofloxacin 5 × 10-5M 6.9 7.9 7.1 3.6 4.1 3.0 2.3 2.5 3.0 52.4 (0.080) 73.3 (0.040)
Vancomycin 5 × 10-5M 6.4 7.4 6.5 3.6 3.6 2.8 2.4 1.6 2.1 66.7 (0.009) 83.7 (0.022)
Gentamycin 5 × 10-5M 7.6 5.5 6.3 2.8 2.6 0.8 1.5 1.0 1.6 74.6 (0.005) 83.7 (0.017)
Ceftriaxone 5 × 10-5M 7.1 6.8 7.0 2.4 2.0 0.9 0.6 0.6 0.7 88.9 (<0.001) 90.7 (0.011)
cTriclosan 5 × 10-5M 6.9 4.9 0.4 0.1 0.0 0.0 0.0 0.0 0.0 100.0 (ND) 98.8 (ND)
cCerulenin 5 × 10-5M 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.0 (ND) 100.0 (ND)
  1. a. Day 40 results based on 1 worm; 5 worms lost to microbial contamination
  2. b. Day 40 results based on 2 worms; 4 worms lost to microbial contamination
  3. c. Denotes compounds that were toxic to the monkey kidney cell feeder layer
  4. The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.