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Table 3 Summary of long-term trial 3: parasite mean motility scores and viability (MTT colorimetry)

From: Onchocerca parasites and Wolbachia endosymbionts: evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro

Compound/drug and conc. Mean motility scores over a period of 40 days Motility MTT
DAY 1 5 10 15 20 25 30 35 40 % reduction on day 40 (P) % inhibition on day 40 (P)
Control 8.4 8.3 7.6 7.5 7.8 7.7 7.6 7.2 7.2   
CGP 6140 1.25 × 10-5M (positive control) 2.5 0.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.0 (<0.001) 67.4 (0.0032)
Rifampicin 5 × 10-5M 8.0 6.6 2.1 0.4 0.4 0.0 0.0 0.0 0.0 100.0 (<0.001) 67.4 (<0.001)
Erythromycin 5 × 10-5M 8.4 8.5 8.0 7.6 7.7 7.6 7.4 7.5 7.4 0.0 (0.539) 0.0 (0.210)
Oxytetracycline 5 × 10-5M 8.0 8.1 7.7 7.5 7.6 7.5 7.1 6.4 6.9 4.2 (0.360) 0.0 (0.995)
Isoniazid 5 × 10-5M 8.2 8.2 7.7 8.0 7.7 7.3 6.8 5.7 5.2 27.8 (0.010) 35.5 (0.074)
Isoniazid 5 × 10-5M + Rifampicin 5 × 10-5M 7.8 8.2 5.0 4.0 2.7 0.8 0.0 0.0 0.0 100.0 (<0.001) 71.7 (0.002)
Trimethoprim 5 × 10-5M 8.4 8.4 7.9 7.6 7.6 8.0 7.5 7.0 7.0 2.8 (0.549) 0.0 (0.319)
Sulphamethoxazole 5 × 10-5M 8.1 8.3 8.4 7.6 7.6 8.0 7.5 7.3 7.1 1.4 (0.848) 0.0 (0.998)
Trimeth. 5 × 10-5M + Sulphamethox. 5 × 10-5M 8.5 8.4 8.0 7.5 6.8 7.5 7.1 7.4 6.9 4.2 (0.449) 0.0 (0.828)
Clofazimine 1.25 × 10-5M 7.7 6.5 5.2 3.5 4.3 3.5 3.2 0.8 0.0 100.0 (<0.001) 77.4 (<0.001)
Clofazimine 3.13 × 10-6M 8.2 7.0 4.5 5.5 5.0 4.8 3.4 1.2 0.8 88.9 (<0.001) 53.3 (0.028)
  1. The effects of exposure to a range of antibiotics and antibiotic combinations on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.
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Filaria Journal

ISSN: 1475-2883